PafR, a novel transcription regulator, is important for pathogenesis in uropathogenic Escherichia coli.

ABSTRACT: The metV genomic island in the chromosome of uropathogenic Escherichia coli (UPEC) encodes a putative transcription factor and a sugar permease of the phosphotransferase system (PTS), which are predicted to compose a Bgl-like sensory system. The presence of these two genes, hereby termed pafR and pafP, respectively, has been previously shown to correlate with isolates causing clinical syndromes. We show here that deletion of both genes impairs the ability of the resulting mutant to infect the CBA/J mouse model of ascending urinary tract infection compared to that of the parent strain, CFT073. Expressing the two genes in trans in the two-gene knockout mutant complemented full virulence. Deletion of either gene individually generated the same phenotype as the double knockout, indicating that

SUBMITTER: Baum M 

PROVIDER: S-EPMC4187875 | biostudies-literature | 2014 Oct

REPOSITORIES: biostudies-literature