Expression of symptoms, viral coat protein and silencing suppressor gene during mixed infection of a N-Wi strain of potato virus Y and an asymptomatic strain of potato virus X.

ABSTRACT: Potato virus Y (PVY) and potato virus X (PVX), the RNA viruses of two different genera results into synergistic interactions on mixed infection. In this study, a N-Wi strain of PVY and a PVX strain that is asymptomatic on potato were used to study their interactions during mixed infection in Nicotiana benthamiana and Nicotiana tabacum with reference to symptom expression, level of coat protein (CP) using ELISA and suppressor gene using real time PCR under high temperature (26-40 °C) and low temperature (5-25 °C) conditions. Both mixed and single infection caused severe necrosis and death of N. benthamiana plants. Single infection of these viruses in N. tabacum showed mild symptoms but mixed infection caused more severe symptoms. Synergistic symptoms were more pronounced under low temperatu

SUBMITTER: Senanayake DM 

PROVIDER: S-EPMC4188204 | biostudies-literature | 2014

REPOSITORIES: biostudies-literature