High sensitivity combined with extended structural coverage of labile compounds via nanoelectrospray ionization at subambient pressures.

ABSTRACT: Subambient pressure ionization with nanoelectrospray (SPIN) has proven to be effective in producing ions with high efficiency and transmitting them to low pressures for increased sensitivity in mass spectrometry (MS) analysis. Here we present evidence that the SPIN source not only improves MS sensitivity but also facilitates the detection of more labile compounds. The gentleness of conventional heated capillary electrospray ionization (ESI) and the SPIN designs was compared in conjunction with the liquid chromatography mass spectrometry (LC-MS) analysis of colominic acid and N-glycans containing sialic acid. Prior experiments conducted with the SPIN interface demonstrated the ability to detect labile glycans such as heavily sialylated and polysialic acid N-glycans, which are difficult to d

SUBMITTER: Cox JT 

PROVIDER: S-EPMC4188276 | biostudies-literature | 2014 Oct

REPOSITORIES: biostudies-literature