Stanniocalcin-1 is induced by hypoxia inducible factor in rat alveolar epithelial cells.

ABSTRACT: Alveolar type II (ATII) cells remain differentiated and express surfactant proteins when cultured at an air-liquid (A/L) interface. When cultured under submerged conditions, ATII cells dedifferentiate and change their gene expression profile. We have previously shown that gene expression under submerged conditions is regulated by hypoxia inducible factor (HIF) signaling due to focal hypoxia resulting from ATII cell metabolism. Herein, we sought to further define gene expression changes in ATII cells cultured under submerged conditions. We performed a genome wide microarray on RNA extracted from rat ATII cells cultured under submerged conditions for 24-48h after switching from an A/L interface. We found significant alterations in gene expression, including upregulation of the HIF target gen

SUBMITTER: Ito Y 

PROVIDER: S-EPMC4188510 | biostudies-literature | 2014 Oct

REPOSITORIES: biostudies-literature