Development and validation of an Haemophilus influenzae supragenome hybridization (SGH) array for transcriptomic analyses.

ABSTRACT: We previously carried out the design and testing of a custom-built Haemophilus influenzae supragenome hybridization (SGH) array that contains probe sequences to 2,890 gene clusters identified by whole genome sequencing of 24 strains of H. influenzae. The array was originally designed as a tool to interrogate the gene content of large numbers of clinical isolates without the need for sequencing, however, the data obtained is quantitative and is thus suitable for transcriptomic analyses. In the current study RNA was extracted from H. influenzae strain CZ4126/02 (which was not included in the design of the array) converted to cDNA, and labelled and hybridized to the SGH arrays to assess the quality and reproducibility of data obtained from these custom-designed chips to serve as a tool for tr

SUBMITTER: Janto BA 

PROVIDER: S-EPMC4188559 | biostudies-literature | 2014

REPOSITORIES: biostudies-literature