Expansion and somatic hypermutation of B-cell clones in rejected human kidney grafts.

ABSTRACT:

Background

B-cell infiltrates are common in rejected kidney allografts, yet their composition is still unclear. The aim of our study was to characterize the clonal composition of B-cell infiltrates of rejected human kidney grafts.

Methods

We used a molecular approach to characterize the partial B-cell repertoires of 5 failed human kidney grafts with detectable B-cell infiltrates. A comparison between the intragraft and blood repertoire was also conducted for 1 case.

Results

Redundant sequences were observed in both blood and graft, although the level of clonal amplification was significantly higher for the graft. Somatic hypermutations (SHMs) were also more frequent in sequences found in the graft compared to the blood. The rate of nonsilent mutations was significantl

SUBMITTER: Ferdman J 

PROVIDER: S-EPMC4188704 | biostudies-literature | 2014 Oct

REPOSITORIES: biostudies-literature