Novel approach to identifying the hepatitis B virus pre-S deletions associated with hepatocellular carcinoma.

ABSTRACT:

Aim

To develop a novel non-sequencing method for the detection of hepatitis B virus (HBV) pre-S deletion mutants in HBV carriers.

Methods

The entire region of HBV pre-S1 and pre-S2 was amplified by polymerase chain reaction (PCR). The size of PCR products was subsequently determined by capillary gel electrophoresis (CGE). CGE were carried out in a PACE-MDQ instrument equipped with a UV detector set at 254 nm. The samples were separated in 50 μm ID eCAP Neutral Coated Capillaries using a voltage of 6 kV for 30 min. Data acquisition and analysis were performed using the 32 Karat Software. A total of 114 DNA clones containing different sizes of the HBV pre-S gene were used to determine the accuracy of the CGE method. One hundred and fifty seven hepatocellular carcinoma (HCC) an

SUBMITTER: Zhao ZM 

PROVIDER: S-EPMC4188909 | biostudies-literature | 2014 Oct

REPOSITORIES: biostudies-literature