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Targeting synaptic pathology with a novel affinity mass spectrometry approach.

ABSTRACT: We report a novel strategy for studying synaptic pathology by concurrently measuring levels of four SNARE complex proteins from individual brain tissue samples. This method combines affinity purification and mass spectrometry and can be applied directly for studies of SNARE complex proteins in multiple species or modified to target other key elements in neuronal function. We use the technique to demonstrate altered levels of presynaptic proteins in Alzheimer disease patients and prion-infected mice.

SUBMITTER: Brinkmalm A

PROVIDER: S-EPMC4188988 | biostudies-literature | 2014 Oct

REPOSITORIES: biostudies-literature

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Targeting synaptic pathology with a novel affinity mass spectrometry approach.

Brinkmalm Ann A Brinkmalm Gunnar G Honer William G WG Moreno Julie A JA Jakobsson Joel J Mallucci Giovanna R GR Zetterberg Henrik H Blennow Kaj K Öhrfelt Annika A

Molecular & cellular proteomics : MCP 20140627 10

We report a novel strategy for studying synaptic pathology by concurrently measuring levels of four SNARE complex proteins from individual brain tissue samples. This method combines affinity purification and mass spectrometry and can be applied directly for studies of SNARE complex proteins in multiple species or modified to target other key elements in neuronal function. We use the technique to demonstrate altered levels of presynaptic proteins in Alzheimer disease patients and prion-infected m ...[more]

PMID: 24973420

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