Characterization of functional reprogramming during osteoclast development using quantitative proteomics and mRNA profiling.

ABSTRACT: In addition to forming macrophages and dendritic cells, monocytes in adult peripheral blood retain the ability to develop into osteoclasts, mature bone-resorbing cells. The extensive morphological and functional transformations that occur during osteoclast differentiation require substantial reprogramming of gene and protein expression. Here we employ -omic-scale technologies to examine in detail the molecular changes at discrete developmental stages in this process (precursor cells, intermediate osteoclasts, and multinuclear osteoclasts), quantitatively comparing their transcriptomes and proteomes. The data have been deposited to the ProteomeXchange with identifier PXD000471. Our analysis identified mitochondrial changes, along with several alterations in signaling pathways, as central to

SUBMITTER: An E 

PROVIDER: S-EPMC4188996 | biostudies-literature | 2014 Oct

REPOSITORIES: biostudies-literature