Phosphorylation and arginine methylation mark histone H2A prior to deposition during Xenopus laevis development.

ABSTRACT:

Background

Stored, soluble histones in eggs are essential for early development, in particular during the maternally controlled early cell cycles in the absence of transcription. Histone post-translational modifications (PTMs) direct and regulate chromatin-templated transactions, so understanding the nature and function of pre-deposition maternal histones is essential to deciphering mechanisms of regulation of development, chromatin assembly, and transcription. Little is known about histone H2A pre-deposition modifications nor known about the transitions that occur upon the onset of zygotic control of the cell cycle and transcription at the mid-blastula transition (MBT).

Results

We isolated histones from staged Xenopus laevis oocytes, eggs, embryos, and assembled pronuclei t

SUBMITTER: Wang WL 

PROVIDER: S-EPMC4191874 | biostudies-literature | 2014

REPOSITORIES: biostudies-literature