Complete mapping of substrate translocation highlights the role of LeuT N-terminal segment in regulating transport cycle.

ABSTRACT: Neurotransmitter: sodium symporters (NSSs) regulate neuronal signal transmission by clearing excess neurotransmitters from the synapse, assisted by the co-transport of sodium ions. Extensive structural data have been collected in recent years for several members of the NSS family, which opened the way to structure-based studies for a mechanistic understanding of substrate transport. Leucine transporter (LeuT), a bacterial orthologue, has been broadly adopted as a prototype in these studies. This goal has been elusive, however, due to the complex interplay of global and local events as well as missing structural data on LeuT N-terminal segment. We provide here for the first time a comprehensive description of the molecular events leading to substrate/Na+ release to the postsynaptic cell, in

SUBMITTER: Cheng MH 

PROVIDER: S-EPMC4191883 | biostudies-literature | 2014 Oct

REPOSITORIES: biostudies-literature