Molecular basis of kindlin-2 binding to integrin-linked kinase pseudokinase for regulating cell adhesion.

ABSTRACT: Integrin-linked kinase (ILK) is a distinct intracellular adaptor essential for integrin-mediated cell-extracellular matrix adhesion, cell spreading, and migration. Acting as a major docking platform in focal adhesions, ILK engages many proteins to dynamically link integrins with the cytoskeleton, but the underlying mechanism remains elusive. Here, we have characterized the interaction of ILK with kindlin-2, a key regulator for integrin bidirectional signaling. We show that human kindlin-2 binds to human ILK with high affinity. Using systematic mapping approaches, we have identified a major ILK binding site involving a 20-residue fragment (residues 339-358) in kindlin-2. NMR-based analysis reveals a helical conformation of this fragment that utilizes its leucine-rich surface to recognize th

SUBMITTER: Fukuda K 

PROVIDER: S-EPMC4192489 | biostudies-literature | 2014 Oct

REPOSITORIES: biostudies-literature