Novel strategy for non-targeted isotope-assisted metabolomics by means of metabolic turnover and multivariate analysis.

ABSTRACT: Isotope-labeling is a useful technique for understanding cellular metabolism. Recent advances in metabolomics have extended the capability of isotope-assisted studies to reveal global metabolism. For instance, isotope-assisted metabolomics technology has enabled the mapping of a global metabolic network, estimation of flux at branch points of metabolic pathways, and assignment of elemental formulas to unknown metabolites. Furthermore, some data processing tools have been developed to apply these techniques to a non-targeted approach, which plays an important role in revealing unknown or unexpected metabolism. However, data collection and integration strategies for non-targeted isotope-assisted metabolomics have not been established. Therefore, a systematic approach is proposed to elucidate

SUBMITTER: Nakayama Y 

PROVIDER: S-EPMC4192689 | biostudies-literature | 2014 Aug

REPOSITORIES: biostudies-literature