A reusable electrochemical proximity assay for highly selective, real-time protein quantitation in biological matrices.

ABSTRACT: Rapid and specific quantitation of a variety of proteins over a wide concentration range is highly desirable for biosensing at the point-of-care, in clinical laboratories, and in research settings. Our recently developed electrochemical proximity assay (ECPA) is a target-flexible, DNA-directed, direct-readout protein quantitation method with detection limits in the low femtomolar range, making it particularly amenable to point-of-care detection. However, consistent quantitation in more complex matrices is required at the point-of-care, and improvements in measurement speed are needed for clinical and research settings. Here, we address these concerns with a reusable ECPA, where a gentle regeneration of the surface DNA monolayer (used to capture the proximity complex) is achieved enzymatica

SUBMITTER: Hu J 

PROVIDER: S-EPMC4193296 | biostudies-literature | 2014 Jun

REPOSITORIES: biostudies-literature