Evaluation of developmental phenotypes produced by morpholino antisense targeting of a sea urchin Runx gene.

ABSTRACT:

Background

Runx transcription factors are important regulators of metazoan development. The sea urchin Runx gene SpRunt was previously identified as a trans-activator of the CyIIIa actin gene, a differentiation marker of larval aboral ectoderm. Here we extend the functional analysis of SpRunt, using morpholino antisense oligonucleotides (morpholinos) to interfere with SpRunt expression in the embryo.

Results

The developmental effects of four different SpRunt-specific morpholinos were evaluated. The two morpholinos most effective at knocking down SpRunt produce an identical mitotic catastrophe phenotype at late cleavage stage that is an artifact of coincidental mis-targeting to histone mRNA, providing a cautionary example of the insufficiency of two different morpholinos as a

SUBMITTER: Coffman JA 

PROVIDER: S-EPMC419381 | biostudies-literature | 2004 May

REPOSITORIES: biostudies-literature