Identification and functional analysis of 'hypothetical' genes expressed in Haemophilus influenzae.

ABSTRACT: The progress in genome sequencing has led to a rapid accumulation in GenBank submissions of uncharacterized 'hypothetical' genes. These genes, which have not been experimentally characterized and whose functions cannot be deduced from simple sequence comparisons alone, now comprise a significant fraction of the public databases. Expression analyses of Haemophilus influenzae cells using a combination of transcriptomic and proteomic approaches resulted in confident identification of 54 'hypothetical' genes that were expressed in cells under normal growth conditions. In an attempt to understand the functions of these proteins, we used a variety of publicly available analysis tools. Close homologs in other species were detected for each of the 54 'hypothetical' genes. For 16 of them, exact fun

SUBMITTER: Kolker E 

PROVIDER: S-EPMC419445 | biostudies-literature | 2004

REPOSITORIES: biostudies-literature