A high-density genetic map with array-based markers facilitates structural and quantitative trait locus analyses of the common wheat genome.

ABSTRACT: The large genome and allohexaploidy of common wheat have complicated construction of a high-density genetic map. Although improvements in the throughput of next-generation sequencing (NGS) technologies have made it possible to obtain a large amount of genotyping data for an entire mapping population by direct sequencing, including hexaploid wheat, a significant number of missing data points are often apparent due to the low coverage of sequencing. In the present study, a microarray-based polymorphism detection system was developed using NGS data obtained from complexity-reduced genomic DNA of two common wheat cultivars, Chinese Spring (CS) and Mironovskaya 808. After design and selection of polymorphic probes, 13,056 new markers were added to the linkage map of a recombinant inbred mapping

SUBMITTER: Iehisa JC 

PROVIDER: S-EPMC4195500 | biostudies-literature | 2014 Oct

REPOSITORIES: biostudies-literature