The cortical cytoskeletal network and cell-wall dynamics in the unicellular charophycean green alga Penium margaritaceum.

ABSTRACT:

Background and aims

Penium margaritaceum is a unicellular charophycean green alga with a unique bi-directional polar expansion mechanism that occurs at the central isthmus zone prior to cell division. This entails the focused deposition of cell-wall polymers coordinated by the activities of components of the endomembrane system and cytoskeletal networks. The goal of this study was to elucidate the structural organization of the cortical cytoskeletal network during the cell cycle and identify its specific functional roles during key cell-wall developmental events: pre-division expansion and cell division.

Methods

Microtubules and actin filaments were labelled during various cell cycle phases with an anti-tubulin antibody and rhodamine phalloidin, respectively. Chemically indu

SUBMITTER: Ochs J 

PROVIDER: S-EPMC4195542 | biostudies-literature | 2014 Oct

REPOSITORIES: biostudies-literature