Quantification of abdominal fat depots in rats and mice during obesity and weight loss interventions.

ABSTRACT:

Background & aims

Obesity is a leading healthcare issue contributing to metabolic diseases. There is a great interest in non-invasive approaches for quantitating abdominal fat in obese animals and humans. In this work, we propose an automated method to distinguish and quantify subcutaneous and visceral adipose tissues (SAT and VAT) in rodents during obesity and weight loss interventions. We have also investigated the influence of different magnetic resonance sequences and sources of variability in quantification of fat depots.

Materials and methods

High-fat diet fed rodents were utilized for investigating the changes during obesity, exercise, and calorie restriction interventions (N = 7/cohort). Imaging was performed on a 7T Bruker ClinScan scanner using fast spin echo (FSE)

SUBMITTER: Kn BP 

PROVIDER: S-EPMC4195648 | biostudies-literature | 2014

REPOSITORIES: biostudies-literature