Expressed repeat elements improve RT-qPCR normalization across a wide range of zebrafish gene expression studies.

ABSTRACT: The selection and validation of stably expressed reference genes is a critical issue for proper RT-qPCR data normalization. In zebrafish expression studies, many commonly used reference genes are not generally applicable given their variability in expression levels under a variety of experimental conditions. Inappropriate use of these reference genes may lead to false interpretation of expression data and unreliable conclusions. In this study, we evaluated a novel normalization method in zebrafish using expressed repetitive elements (ERE) as reference targets, instead of specific protein coding mRNA targets. We assessed and compared the expression stability of a number of EREs to that of commonly used zebrafish reference genes in a diverse set of experimental conditions including a develop

SUBMITTER: Vanhauwaert S 

PROVIDER: S-EPMC4195698 | biostudies-literature | 2014

REPOSITORIES: biostudies-literature