Measurement of glycosylated alpha-fetoprotein improves diagnostic power over the native form in hepatocellular carcinoma.

ABSTRACT: Serum alpha-fetoprotein (AFP) has long been used as a diagnostic marker for hepatocellular carcinoma (HCC), albeit controversially. Although it remains widely used in clinics, the value of AFP in HCC diagnosis has recently been challenged due to its significant rates of false positive and false negative findings. To improve the efficacy of AFP as HCC diagnostic marker, we developed a method of measuring total and glycosylated AFP by multiple reaction monitoring (MRM)-MS. In this study, we verified the total amount of AFP (nonglycopeptide levels) and the degree of glycosylated AFP (deglycopeptide levels) in 60 normal (41 men and 19 women; mean age 53 years; range 32-74 years), 35 LC (23 men and 12 women; mean age 56 years; range 43-78 years; HBV-related), and 60 HCC subjects (42 men and 18

SUBMITTER: Kim H 

PROVIDER: S-EPMC4195728 | biostudies-literature | 2014

REPOSITORIES: biostudies-literature