High-throughput multiplex HLA genotyping by next-generation sequencing using multi-locus individual tagging.

ABSTRACT:

Background

Unambiguous human leukocyte antigen (HLA) typing is important in transplant matching and disease association studies. High-resolution HLA typing that is not restricted to the peptide-binding region can decrease HLA allele ambiguities. Cost and technology constraints have hampered high-throughput and efficient high resolution unambiguous HLA typing. We have developed a method for HLA genotyping that preserves the very high-resolution that can be obtained by next-generation sequencing (NGS) but also achieves substantially increased efficiency. Unambiguous HLA-A, B, C and DRB1 genotypes can be determined for 96 individuals in a single run of the Illumina MiSeq.

Results

Long-range amplification of full-length HLA genes from four loci was performed in separate polymera

SUBMITTER: Ehrenberg PK 

PROVIDER: S-EPMC4196003 | biostudies-literature | 2014 Oct

REPOSITORIES: biostudies-literature