Clinical significance of phenotyping and karyotyping of circulating tumor cells in patients with advanced gastric cancer.

ABSTRACT:

Background

Karyotyping and phenotyping of circulating tumor cells (CTCs) in therapeutic cancer patients is of particular clinical significance in terms of both identifying chemo-resistant CTC subtypes and understanding CTC evolution.

Methods

The integrated subtraction enrichment (SET) and immunostaining-fluorescence in situ hybridization (iFISH) platform was applied to detect and characterize CTCs in patients with advanced gastric cancer (AGC). Status of human epidermal growth factor receptor 2 (HER2) expressing and aneuploidy of chromosome 8 in CTCs enriched from the patients was examined by SET-iFISH following clinical chemotherapy or HER2-targeted therapy. CellSearch system was applied as a reference control.

Results

Phenotyping of CTCs in HER2 positive AGC patient

SUBMITTER: Li Y 

PROVIDER: S-EPMC4196148 | biostudies-literature | 2014 Aug

REPOSITORIES: biostudies-literature