A comprehensive functional map of the hepatitis C virus genome provides a resource for probing viral proteins.

ABSTRACT:

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Pairing high-throughput sequencing technologies with high-throughput mutagenesis enables genome-wide investigations of pathogenic organisms. Knowledge of the specific functions of protein domains encoded by the genome of the hepatitis C virus (HCV), a major human pathogen that contributes to liver disease worldwide, remains limited to insight from small-scale studies. To enhance the capabilities of HCV researchers, we have obtained a high-resolution functional map of the entire viral genome by combining transposon-based insertional mutagenesis with next-generation sequencing. We generated a library of 8,398 mutagenized HCV clones, each containing one 15-nucleotide sequence inserted at a unique genomic position. We passaged this library in hepatic cells, recovered virus p

SUBMITTER: Remenyi R 

PROVIDER: S-EPMC4196222 | biostudies-literature | 2014 Sep

REPOSITORIES: biostudies-literature