Selective whole genome amplification for resequencing target microbial species from complex natural samples.

ABSTRACT: Population genomic analyses have demonstrated power to address major questions in evolutionary and molecular microbiology. Collecting populations of genomes is hindered in many microbial species by the absence of a cost effective and practical method to collect ample quantities of sufficiently pure genomic DNA for next-generation sequencing. Here we present a simple method to amplify genomes of a target microbial species present in a complex, natural sample. The selective whole genome amplification (SWGA) technique amplifies target genomes using nucleotide sequence motifs that are common in the target microbe genome, but rare in the background genomes, to prime the highly processive phi29 polymerase. SWGA thus selectively amplifies the target genome from samples in which it originally repr

SUBMITTER: Leichty AR 

PROVIDER: S-EPMC4196606 | biostudies-literature | 2014 Oct

REPOSITORIES: biostudies-literature