Fibrillar β-amyloid 1-42 alters cytokine secretion, cholinergic signalling and neuronal differentiation.

ABSTRACT: Adult neurogenesis is impaired by inflammatory processes, which are linked to altered cholinergic signalling and cognitive decline in Alzheimer's disease. In this study, we investigated how amyloid beta (Aβ)-evoked inflammatory responses affect the generation of new neurons from human embryonic stem (hES) cells and the role of cholinergic signalling in regulating this process. The hES were cultured as neurospheres and exposed to fibrillar and oligomeric Aβ(1-42) (Aβf, AβO) or to conditioned medium from human primary microglia activated with either Aβ(1-42) or lipopolysaccharide. The neurospheres were differentiated for 29 days in vitro and the resulting neuronal or glial phenotypes were thereafter assessed. Secretion of cytokines and the enzymes acetylcholinesterase (AChE), butyrylcholines

SUBMITTER: Malmsten L 

PROVIDER: S-EPMC4196662 | biostudies-literature | 2014 Sep

REPOSITORIES: biostudies-literature