The actin regulators Enabled and Diaphanous direct distinct protrusive behaviors in different tissues during Drosophila development.

ABSTRACT: Actin-based protrusions are important for signaling and migration during development and homeostasis. Defining how different tissues in vivo craft diverse protrusive behaviors using the same genomic toolkit of actin regulators is a current challenge. The actin elongation factors Diaphanous and Enabled both promote barbed-end actin polymerization and can stimulate filopodia in cultured cells. However, redundancy in mammals and Diaphanous' role in cytokinesis limited analysis of whether and how they regulate protrusions during development. We used two tissues driving Drosophila dorsal closure--migratory leading-edge (LE) and nonmigratory amnioserosal (AS) cells--as models to define how cells shape distinct protrusions during morphogenesis. We found that nonmigratory AS cells produce filopodi

SUBMITTER: Nowotarski SH 

PROVIDER: S-EPMC4196866 | biostudies-literature | 2014 Oct

REPOSITORIES: biostudies-literature