Fractionation profiling: a fast and versatile approach for mapping vesicle proteomes and protein-protein interactions.

ABSTRACT: We developed "fractionation profiling," a method for rapid proteomic analysis of membrane vesicles and protein particles. The approach combines quantitative proteomics with subcellular fractionation to generate signature protein abundance distribution profiles. Functionally associated groups of proteins are revealed through cluster analysis. To validate the method, we first profiled >3500 proteins from HeLa cells and identified known clathrin-coated vesicle proteins with >90% accuracy. We then profiled >2400 proteins from Drosophila S2 cells, and we report the first comprehensive insect clathrin-coated vesicle proteome. Of importance, the cluster analysis extends to all profiled proteins and thus identifies a diverse range of known and novel cytosolic and membrane-associated protein comple

SUBMITTER: Borner GH 

PROVIDER: S-EPMC4196868 | biostudies-literature | 2014 Oct

REPOSITORIES: biostudies-literature