Whole genome mapping as a fast-track tool to assess genomic stability of sequenced Staphylococcus aureus strains.

ABSTRACT:

Background

Whole genome (optical) mapping (WGM), a state-of-the-art mapping technology based on the generation of high resolution restriction maps, has so far been used for typing clinical outbreak strains and for mapping de novo sequence contigs in genome sequencing projects. We employed WGM to assess the genomic stability of previously sequenced Staphylococcus aureus strains that are commonly used in laboratories as reference standards.

Results

S. aureus strains (n = 12) were mapped on the Argus™ Optical Mapping System (Opgen Inc, Gaithersburg, USA). Assembly of NcoI-restricted DNA molecules, visualization, and editing of whole genome maps was performed employing MapManager and MapSolver softwares (Opgen Inc). In silico whole genome NcoI-restricted maps were also generated

SUBMITTER: Sabirova JS 

PROVIDER: S-EPMC4197248 | biostudies-literature | 2014 Oct

REPOSITORIES: biostudies-literature