Introduction of impermeable actin-staining molecules to mammalian cells by optoporation.

ABSTRACT: The selective insertion of foreign materials, such as fluorescent markers or plasmids, into living cells has been a challenging problem in cell biology due to the cell membrane's selective permeability. However, it is often necessary that researchers insert such materials into cells for various dynamical and/or drug delivery studies. This problem becomes even more challenging if the study is to be limited to specific cells within a larger population, since other transfection methods, such as viral transfection and lipofection, are not realizable with a high degree of spatial selectivity. Here, we have used a focused femtosecond laser beam to create a small transient hole in the cellular membrane (optoporation) in order to inject nanomolar concentrations of rhodamine phalloidin (an impermea

SUBMITTER: Dhakal K 

PROVIDER: S-EPMC4197434 | biostudies-literature | 2014 Oct

REPOSITORIES: biostudies-literature