BDNF and the maturation of posttranscriptional regulatory networks in human SH-SY5Y neuroblast differentiation.

ABSTRACT: The SH-SY5Y culture system is a convenient neuronal model with the potential to elaborate human/primate-specific transcription networks and pathways related to human cognitive disorders. While this system allows for the exploration of specialized features in the human genome, there is still significant debate about how this model should be implemented, and its appropriateness for answering complex functional questions related to human neural architecture. In view of these questions we sought to characterize the posttranscriptional regulatory structure of the two-stage ATRA differentiation, BDNF maturation protocol proposed by Encinas et al. (2000) using integrative whole-genome gene and microRNA (miRNA) expression analysis. We report that ATRA-BDNF induced significant increases in expressi

SUBMITTER: Goldie BJ 

PROVIDER: S-EPMC4197648 | biostudies-literature | 2014

REPOSITORIES: biostudies-literature