Molecular mechanism of formation of cortical opacity in CRYAAN101D transgenic mice.

ABSTRACT:

Purpose

The CRYAAN101D transgenic mouse model expressing deamidated αA-crystallin (deamidation at N101 position to D) develops cortical cataract at the age of 7 to 9 months. The present study was carried out to explore the molecular mechanism that leads to the development of cortical opacity in CRYAAN101D lenses.

Methods

RNA sequence analysis was carried out on 2- and 4-month-old αA-N101D and wild type (WT) lenses. To understand the biologic relevance and function of significantly altered genes, Ingenuity Pathway Analysis (IPA) was done. To elucidate terminal differentiation defects, immunohistochemical, and Western blot analyses were carried out.

Results

RNA sequence and IPA data suggested that the genes belonging to gene expression, cellular assembly and organizatio

SUBMITTER: Hegde SM 

PROVIDER: S-EPMC4197685 | biostudies-literature | 2014 Aug

REPOSITORIES: biostudies-literature