Translational profiling through biotinylation of tagged ribosomes in zebrafish.

ABSTRACT: Heterogeneity within a population of cells of the same type is a common theme in metazoan biology. Dissecting complex developmental and physiological processes crucially relies on our ability to probe the expression profile of these cell subpopulations. Current strategies rely on cell enrichment based on sequential or simultaneous use of multiple intersecting markers starting from a heterogeneous cell suspension. The extensive tissue manipulations required to generate single-cell suspensions, as well as the complexity of the required equipment, inherently complicate these approaches. Here, we propose an alternative methodology based on a genetically encoded system in the model organism Danio rerio (zebrafish). In transgenic fish, we take advantage of the combinatorial biotin transfer syste

SUBMITTER: Housley MP 

PROVIDER: S-EPMC4197690 | biostudies-literature | 2014 Oct

REPOSITORIES: biostudies-literature