Exclusive multipotency and preferential asymmetric divisions in post-embryonic neural stem cells of the fish retina.

ABSTRACT: The potency of post-embryonic stem cells can only be addressed in the living organism, by labeling single cells after embryonic development and following their descendants. Recently, transplantation experiments involving permanently labeled cells revealed multipotent neural stem cells (NSCs) of embryonic origin in the medaka retina. To analyze whether NSC potency is affected by developmental progression, as reported for the mammalian brain, we developed an inducible toolkit for clonal labeling and non-invasive fate tracking. We used this toolkit to address post-embryonic stem cells in different tissues and to functionally differentiate transient progenitor cells from permanent, bona fide stem cells in the retina. Using temporally controlled clonal induction, we showed that post-embryonic r

SUBMITTER: Centanin L 

PROVIDER: S-EPMC4197724 | biostudies-literature | 2014 Sep

REPOSITORIES: biostudies-literature