Generation and screening of a comprehensive Mycobacterium avium subsp. paratuberculosis transposon mutant bank.

ABSTRACT: Mycobacterium avium subsp. paratuberculosis (MAP) is the etiologic agent of Johne's Disease in ruminants. This enteritis has significant economic impact and worldwide distribution. Vaccination is one of the most cost effective infectious disease control measures. Unfortunately, current vaccines reduce clinical disease and shedding, but are of limited efficacy and do not provide long-term protective immunity. Several strategies have been followed to mine the MAP genome for virulence determinants that could be applied to vaccine and diagnostic assay development. In this study, a comprehensive mutant bank of 13,536 MAP K-10 Tn5367 mutants (P > 95%) was constructed and screened in vitro for phenotypes related to virulence. This strategy was designated to maximize identification of genes import

SUBMITTER: Rathnaiah G 

PROVIDER: S-EPMC4197770 | biostudies-literature | 2014

REPOSITORIES: biostudies-literature