Characteristic bimodal profiles of RNA polymerase II at thousands of active mammalian promoters.

ABSTRACT:

Background

In mammals, ChIP-seq studies of RNA polymerase II (PolII) occupancy have been performed to reveal how recruitment, initiation and pausing of PolII may control transcription rates, but the focus is rarely on obtaining finely resolved profiles that can portray the progression of PolII through sequential promoter states.

Results

Here, we analyze PolII binding profiles from high-coverage ChIP-seq on promoters of actively transcribed genes in mouse and humans. We show that the enrichment of PolII near transcription start sites exhibits a stereotypical bimodal structure, with one peak near active transcription start sites and a second peak 110 base pairs downstream from the first. Using an empirical model that reliably quantifies the spatial PolII signal, gene by gene,

SUBMITTER: Quinodoz M 

PROVIDER: S-EPMC4197824 | biostudies-literature | 2014 Jun

REPOSITORIES: biostudies-literature