Active enhancers are delineated de novo during hematopoiesis, with limited lineage fidelity among specified primary blood cells.

ABSTRACT: Tissues may adopt diverse strategies to establish specific transcriptional programs in daughter lineages. In intestinal crypts, enhancers for genes expressed in both major cell types appear broadly permissive in stem and specified progenitor cells. In blood, another self-renewing tissue, it is unclear when chromatin becomes permissive for transcription of genes expressed in distinct terminal lineages. Using chromatin immunoprecipitation (ChIP) combined with deep sequencing (ChIP-seq) to profile activating histone marks, we studied enhancer dynamics in primary mouse blood stem, progenitor, and specified cells. Stem and multipotent progenitor cells show scant H3K4me2 marking at enhancers bound by specific transcription factors in their committed progeny. Rather, enhancers are modulated dynam

SUBMITTER: Luyten A 

PROVIDER: S-EPMC4197967 | biostudies-literature | 2014 Aug

REPOSITORIES: biostudies-literature