Mimotope-based vaccines of Leishmania infantum antigens and their protective efficacy against visceral leishmaniasis.

ABSTRACT:

Background

The development of cost-effective prophylactic strategies to prevent leishmaniasis has become a high-priority. The present study has used the phage display technology to identify new immunogens, which were evaluated as vaccines in the murine model of visceral leishmaniasis (VL). Epitope-based immunogens, represented by phage-fused peptides that mimic Leishmania infantum antigens, were selected according to their affinity to antibodies from asymptomatic and symptomatic VL dogs' sera.

Methodology/main findings

Twenty phage clones were selected after three selection cycles, and were evaluated by means of in vitro assays of the immune stimulation of spleen cells derived from naive and chronically infected with L. infantum BALB/c mice. Clones that were able to induce s

SUBMITTER: Costa LE 

PROVIDER: S-EPMC4198211 | biostudies-literature | 2014

REPOSITORIES: biostudies-literature