Independent effects of sham laparotomy and anesthesia on hepatic microRNA expression in rats.

ABSTRACT:

Background

Studies on liver regeneration following partial hepatectomy (PH) have identified several microRNAs (miRNAs) that show a regulated expression pattern. These studies involve major surgery to access the liver, which is known to have intrinsic effects on hepatic gene expression and may also affect miRNA screening results. We performed two-third PH or sham laparotomy (SL) in Wistar rats to investigate the effect of both procedures on miRNA expression in liver tissue and corresponding plasma samples by microarray and qRT-PCR analyses. As control groups, non-treated rats and rats undergoing anesthesia only were used.

Results

We found that 49 out of 323 miRNAs (15%) were significantly deregulated after PH in liver tissue 12 to 48 hours postoperatively (>20% change), while

SUBMITTER: Werner W 

PROVIDER: S-EPMC4198680 | biostudies-literature | 2014 Oct

REPOSITORIES: biostudies-literature