Knockout of an outer membrane protein operon of Anaplasma marginale by transposon mutagenesis.

ABSTRACT:

Background

The large amounts of data generated by genomics, transcriptomics and proteomics have increased our understanding of the biology of Anaplasma marginale. However, these data have also led to new assumptions that require testing, ideally through classical genetic mutation. One example is the definition of genes associated with virulence. Here we describe the molecular characterization of a red fluorescent and spectinomycin and streptomycin resistant A. marginale mutant generated by Himar1 transposon mutagenesis.

Results

High throughput genome sequencing to determine the Himar1-A. marginale genome junctions established that the transposon sequences were integrated within the coding region of the omp10 gene. This gene is arranged within an operon with AM1225 at the 5'

SUBMITTER: Crosby FL 

PROVIDER: S-EPMC4198910 | biostudies-literature | 2014 Apr

REPOSITORIES: biostudies-literature