Evaluation of immunomagnetic separation for the detection of Salmonella in surface waters by polymerase chain reaction.

ABSTRACT: Salmonella spp. is associated with fecal pollution and capable of surviving for long periods in aquatic environments. Instead of the traditional, time-consuming biochemical detection, polymerase chain reaction (PCR) allows rapid identification of Salmonella directly concentrated from water samples. However, prevalence of Salmonella may be underestimated because of the vulnerability of PCR to various environmental chemicals like humic acid, compounded by the fact that various DNA polymerases have different susceptibility to humic acid. Because immunomagnetic separation (IMS) theoretically could isolate Salmonella from other microbes and facilitate removal of aquatic PCR inhibitors of different sizes, this study aims to compare the efficiency of conventional PCR combined with immunomagnetic

SUBMITTER: Hsu CY 

PROVIDER: S-EPMC4199051 | biostudies-literature | 2014 Sep

REPOSITORIES: biostudies-literature