The effects of temperature and anesthetic agents on ciliary function in murine respiratory epithelia.

ABSTRACT:

Background

Mucus transport mediated by motile cilia in the airway is an important defense mechanism for prevention of respiratory infections. As cilia motility can be depressed by hypothermia or exposure to anesthetics, in this study, we investigated the individual and combined effects of dexmedetomidine (dex), fentanyl (fen), and/or isoflurane (iso) at physiologic and low temperatures on cilia motility in mouse tracheal airway epithelia. These anesthetic combinations and low temperature conditions are often used in the setting of cardiopulmonary bypass surgery, surgical repair of congenital heart disease, and cardiac intensive care.

Methods

C57BL/6J mouse tracheal epithelia were excised and cilia dynamics were captured by videomicroscopy following incubation at 15, 22-24, a

SUBMITTER: Christopher AB 

PROVIDER: S-EPMC4199259 | biostudies-literature | 2014

REPOSITORIES: biostudies-literature