An integrated cell purification and genomics strategy reveals multiple regulators of pancreas development.

ABSTRACT: The regulatory logic underlying global transcriptional programs controlling development of visceral organs like the pancreas remains undiscovered. Here, we profiled gene expression in 12 purified populations of fetal and adult pancreatic epithelial cells representing crucial progenitor cell subsets, and their endocrine or exocrine progeny. Using probabilistic models to decode the general programs organizing gene expression, we identified co-expressed gene sets in cell subsets that revealed patterns and processes governing progenitor cell development, lineage specification, and endocrine cell maturation. Purification of Neurog3 mutant cells and module network analysis linked established regulators such as Neurog3 to unrecognized gene targets and roles in pancreas development. Iterative modu

SUBMITTER: Benitez CM 

PROVIDER: S-EPMC4199491 | biostudies-literature | 2014 Oct

REPOSITORIES: biostudies-literature