Characterization and functional analysis of the poxB gene, which encodes pyruvate oxidase in Lactobacillus plantarum.

ABSTRACT: The pyruvate oxidase gene (poxB) from Lactobacillus plantarum Lp80 was cloned and characterized. Northern blot and primer extension analyses revealed that transcription of poxB is monocistronic and under the control of a vegetative promoter. poxB mRNA expression was strongly induced by aeration and was repressed by glucose. Moreover, Northern blotting performed at different stages of growth showed that poxB expression is maximal in the early stationary phase when glucose is exhausted. Primer extension and in vivo footprint analyses revealed that glucose repression of poxB is mediated by CcpA binding to the cre site identified in the promoter region. The functional role of the PoxB enzyme was studied by using gene overexpression and knockout in order to evaluate its implications for acetate

SUBMITTER: Lorquet F 

PROVIDER: S-EPMC419957 | biostudies-literature | 2004 Jun

REPOSITORIES: biostudies-literature