The glycosphingolipid P₁ is an ovarian cancer-associated carbohydrate antigen involved in migration.

ABSTRACT:

Background

The level of plasma-derived naturally circulating anti-glycan antibodies (AGA) to P1 trisaccharide has previously been shown to significantly discriminate between ovarian cancer patients and healthy women. Here we aim to identify the Ig class that causes this discrimination, to identify on cancer cells the corresponding P1 antigen recognised by circulating anti-P1 antibodies and to shed light into the possible function of this glycosphingolipid.

Methods

An independent Australian cohort was assessed for the presence of anti-P1 IgG and IgM class antibodies using suspension array. Monoclonal and human derived anti-glycan antibodies were verified using three independent glycan-based immunoassays and flow cytometry-based inhibition assay. The P1 antigen was detected by

SUBMITTER: Jacob F 

PROVIDER: S-EPMC4200095 | biostudies-literature | 2014 Oct

REPOSITORIES: biostudies-literature