Transcriptome sequencing of rhizome tissue of Sinopodophyllum hexandrum at two temperatures.

ABSTRACT:

Background

Sinopodophyllum hexandrum is an endangered medicinal herb, which is commonly present in elevations ranging between 2,400-4,500 m and is sensitive to temperature. Medicinal property of the species is attributed to the presence of podophyllotoxin in the rhizome tissue. The present work analyzed transcriptome of rhizome tissue of S. hexandrum exposed to 15°C and 25°C to understand the temperature mediated molecular responses including those associated with podophyllotoxin biosynthesis.

Results

Deep sequencing of transcriptome with an average coverage of 88.34X yielded 60,089 assembled transcript sequences representing 20,387 unique genes having homology to known genes. Fragments per kilobase of exon per million fragments mapped (FPKM) based expression analysis reveal

SUBMITTER: Kumari A 

PROVIDER: S-EPMC4200142 | biostudies-literature | 2014 Oct

REPOSITORIES: biostudies-literature