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In vitro and in vivo characterization of a West Nile virus MAD78 infectious clone.

ABSTRACT: The viral determinants governing the varied neuropathogenicity of different West Nile virus (WNV) strains are poorly understood. Here, we generated an infectious clone (WNV-MAD(IC)) of the non-pathogenic strain WNV-MAD78 and compared its replication to that of parental WNV-MAD78 and a WNV-MAD78 infectious clone (WNV-MAD(TX-UTRs)) containing the 5' and 3' untranslated regions (UTRs) of the pathogenic strain WNV-TX. All three viruses replicated at similar rates and caused similar lethality in mice. Thus, the infectious clone is indistinguishable from parental virus in replication and neurovirulence, and the UTRs alone do not account for the increased virulence of WNV-TX compared to WNV-MAD78.

SUBMITTER: Hussmann KL

PROVIDER: S-EPMC4200346 | biostudies-literature | 2014 Nov

REPOSITORIES: biostudies-literature

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In vitro and in vivo characterization of a West Nile virus MAD78 infectious clone.

Hussmann Katherine L KL Vandergaast Rianna R Ochsner Susan Park SP Huang Albert C AC Gale Michael M Fredericksen Brenda L BL

Archives of virology 20140715 11

The viral determinants governing the varied neuropathogenicity of different West Nile virus (WNV) strains are poorly understood. Here, we generated an infectious clone (WNV-MAD(IC)) of the non-pathogenic strain WNV-MAD78 and compared its replication to that of parental WNV-MAD78 and a WNV-MAD78 infectious clone (WNV-MAD(TX-UTRs)) containing the 5' and 3' untranslated regions (UTRs) of the pathogenic strain WNV-TX. All three viruses replicated at similar rates and caused similar lethality in mice ...[more]

PMID: 25023336

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