Su(H)-mediated repression positions gene boundaries along the dorsal-ventral axis of Drosophila embryos.

ABSTRACT: In Drosophila embryos, a nuclear gradient of the Dorsal (Dl) transcription factor directs differential gene expression along the dorsoventral (DV) axis, translating it into distinct domains that specify future mesodermal, neural, and ectodermal territories. However, the mechanisms used to differentially position gene expression boundaries along this axis are not fully understood. Here, using a combination of approaches, including mutant phenotype analyses and chromatin immunoprecipitation, we show that the transcription factor Suppressor of Hairless, Su(H), helps define dorsal boundaries for many genes expressed along the DV axis. Synthetic reporter constructs also provide molecular evidence that Su(H) binding sites support repression and act to counterbalance activation through Dl and the

SUBMITTER: Ozdemir A 

PROVIDER: S-EPMC4201238 | biostudies-literature | 2014 Oct

REPOSITORIES: biostudies-literature