Improved methodical approach for quantitative BRET analysis of G Protein Coupled Receptor dimerization.

ABSTRACT: G Protein Coupled Receptors (GPCR) can form dimers or higher ordered oligomers, the process of which can remarkably influence the physiological and pharmacological function of these receptors. Quantitative Bioluminescence Resonance Energy Transfer (qBRET) measurements are the gold standards to prove the direct physical interaction between the protomers of presumed GPCR dimers. For the correct interpretation of these experiments, the expression of the energy donor Renilla luciferase labeled receptor has to be maintained constant, which is hard to achieve in expression systems. To analyze the effects of non-constant donor expression on qBRET curves, we performed Monte Carlo simulations. Our results show that the decrease of donor expression can lead to saturation qBRET curves even if the int

SUBMITTER: Szalai B 

PROVIDER: S-EPMC4201472 | biostudies-literature | 2014

REPOSITORIES: biostudies-literature