Caspase cleavage sites in the human proteome: CaspDB, a database of predicted substrates.

ABSTRACT: Caspases are enzymes belonging to a conserved family of cysteine-dependent aspartic-specific proteases that are involved in vital cellular processes and play a prominent role in apoptosis and inflammation. Determining all relevant protein substrates of caspases remains a challenging task. Over 1500 caspase substrates have been discovered in the human proteome according to published data and new substrates are discovered on a daily basis. To aid the discovery process we developed a caspase cleavage prediction method using the recently published curated MerCASBA database of experimentally determined caspase substrates and a Random Forest classification method. On both internal and external test sets, the ranking of predicted cleavage positions is superior to all previously developed predicti

SUBMITTER: Kumar S 

PROVIDER: S-EPMC4201543 | biostudies-literature | 2014

REPOSITORIES: biostudies-literature